To characterize the pluripotency of iPSC, a class of defined tests can be used, and usually iPSC are characterized with the criteria applied to ES cells. Due to the differences between iPSC and ES cell expression patterns, their characteristics also not exactly the same. There are a variety of features assessed by characterization, such as cell and colony morphology, the expression of surface markers and other antigens, growth rate, the expression of pluripotency marker genes, enzyme levels, methylation statuses, as well as differentiation into somatic cell types in vitro and in vivo. Besides, among numerous pluripotency markers, OCT3/4 and Nanog are considered to be archetypal pluripotent stem cell markers. Moreover, it has demonstrated that OCT3/4 and SOX2 work together via feedback loops regulating both their own transcription and other pluripotency-associated genes.
Check more details: Cell line Characterization and Pluripotency
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